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www.benisonmedia.com  ARTICLE    feed intake compared to the   4th week compared to other   performance and FCR when      Fig. 3: Effect of dietary supplementation with different fractions of FWP on FCR of broiler chickens [Values with   www.benisonmedia.com

                                                                                                                                 Think Grain Think Feed - Volume 11 | Issue 9 | July 2025
                                                                                 Think Grain Think Feed - Volume 11 | Issue 9 | July 2025


                                                               treatment groups (p < 0.05)
                                      control. This improved
                                                                                                                               different superscripts differ significantly (p<0.05), Mean±SD, n=4].
                                                                                         whey was combined with
                                                               as shown in Fig. 3. While no
                                                                                         probiotics. Kanza et al. (2019)
                                      efficiency is attributed to
                                                               significant difference in FCR
                                                                                         noted that whey protein
                                      FWP's superior nutritional
                                      profile, offering high-quality
                                                               study period, the T1 group
                                                                                         supplementation boosted
                                      protein, essential amino
                                                               maintained the highest FCR
                                      acids, and branched-chain   was observed over the entire   concentrate (WPC)
                                                                                         feed consumption, weight
                                      amino acids (BCAAs), known   (1.95) at the 6th week,   gain, and body weight,
                                      for their role in muscle   numerically higher than T2   though without significantly
                                      protein metabolism and   (1.78), T4 (1.67), and T3   affecting FCR. Ashour et al.
                                      overall growth. Bovine whey   (1.67). This higher FCR in the   (2020) demonstrated
                                      protein's balanced essential   control group was linked to   improved FCR in all WPC-fed
                                      amino acid composition,   its higher feed consumption.   broiler groups compared to
                                      enhanced digestibility, and   The lower FCR observed in   controls, with optimal results
                                      high biological value    FWP-fed groups is         at 0.20% and 0.25% WPC.
                                      contribute to this. Beyond   potentially due to the   While FWP generally
                                      providing amino acids, FWP's  satiety-inducing effects of   promote satiety, enhance
                                      bioactive peptides (2–20   whey-derived peptides,   growth performance, and
                                      amino acid residues) exert   leading to reduced overall   support overall health and
                                      antimicrobial, antioxidant,   feed intake.         productivity, a crucial point         broilers.                 reduced serum triglyceride,   density lipoprotein (HDL),
                                      antihypertensive, and    FWPs offer multiple       of our study was to directly          Supplementation with      cholesterol, and total lipid   low-density lipoprotein
                                      immunomodulatory effects,   functional benefits beyond   evaluate fermented whey         fermented cheese whey     levels in broiler chickens,   (LDL), and very-low-density
                                      promoting intestinal health   those of typical prebiotics   peptides (FWPs) as a viable   fractions (FWP) significantly   while no significant effect   lipoprotein (VLDL) levels over
                                      and disease resistance,   and probiotics, which    alternative to antibiotics in                                   was observed on high-     the entire study period as
                                      thereby enhancing feed   primarily support gut     poultry. We found that FWPs,
                                      efficiency. The T4 group,   immunity. FWP contains   administered for a mere one
                                      specifically, showed     essential amino acids,    week (1 mL per day), yielded
                                      enhanced gut development   organic acids, minerals,   comparable effects on
                                      and accelerated muscle   vitamins, and bioactive whey   growth performance to a
                                      growth, resulting in the   peptides with antioxidant   conventional probiotic and
                                      highest observed body    and antidiabetic properties.   immunomodulator regimen
                                      weight gain. All FWP diet   This multi-faceted     maintained for 42 days. This
                                      groups demonstrated      functionality contributes to   striking efficiency
                                      increased feed consumption   enhanced immunity,    underscores not only the
                                      and body weight gain until   improved gut microflora, and   health benefits of FWP for
                                      the 6th week, with FWP diets   a better blood profile in the   broiler birds, but also
                                      proving more palatable than   long term.           significant potential
                                      the standard diet.       Similar studies support these   economic advantages for
                                      Importantly, the addition of   findings: Hilmi et al. (2018)   poultry farmers due to
                                      whey protein fractions did   reported that fermented   reduced administration
                                      not affect the mortality rate   cheese whey as a   duration. This aligns with the
                                      in any FWP treatment groups   nutraceutical significantly   broader consumer demand
                                      (T2, T3, T4). The >10 kDa   decreased feed consumption   for antibiotic-free poultry,
                                      FWP fraction (T4) particularly   and FCR in poultry, while   which offers a reduced risk of
                                      improved overall body    also increasing egg       antimicrobial-resistant (AMR)
                                      weight gain by 42 days of   production. While some   pathogens and improved gut
                                      age.                     research, like Sugiharto et al.   health in the final product.
                                      Regarding Feed Conversion   (2019), found no significant   Future research will further
                                      Ratio (FCR), a key indicator of  impact of whey powder on   explore FWP's impact on
                                      feed quality and weight gain   broiler FCR, others, such as   body fat composition, meat
                                      efficiency, the control group   Samli et al. (2019) and Zarei   and egg quality, egg
                                      (T1) exhibited a significantly   et al. (2020), observed   production capacity, and
                                      higher average FCR at the   improved growth        meat protein content in



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