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www.benisonmedia.com ARTICLE Think Grain Think Feed - Volume 11 | Issue 9 | July 2025 as a nutraceutical + commercial probiotics + DNA stool kit (Qiagen), www.benisonmedia.com

Think Grain Think Feed - Volume 11 | Issue 9 | July 2025

supplement to basal diets,
quantified by Qubit 3.0
immunomodulator for 42
days); T2 (basal diet + <3
analyzing its effects on
fluorometer, and quality
growth performance, blood
checked by agarose gel
kDa FWP for 7 days); T3
parameters, relative organs
for 7 days); T4 (basal diet +
(via histological analysis),
amplicon sequencing
and metagenomic profiles of (basal diet + <10 kDa FWP electrophoresis. 16S
targeting the V3-V4 region
>10 kDa FWP for 7 days).
broiler cecal contents, aiming Freshly prepared FWP (1 mL) was performed using 341F
to establish it as a viable, was orally administered daily and 785R primers, following
biohazard-free substitute for from day 8 to day 15. Basal Illumina 16S Metagenomics
antibiotics in organic poultry diets, formulated from corn library preparation
production. and soybean mash per Hati guidelines. Sequencing data
Material and Method et al. (2020) and Table S1, was analyzed in R v4.2.2
met broiler starter, grower, using DADA2 package
This study investigated the
and finisher nutritional v1.26.0 for ASV generation
effects of fermented whey
requirements and were and taxonomic assignment,
peptides (FWP) on broiler
provided ad libitum. followed by phyloseq R
chickens. Cheese whey,
sourced from Vidya Dairy, Growth performance (BW, FI, package v1.46.0 for
Anand, India, was sterilized BWG, FCR) was assessed microbiome analysis, with
Scope of Fermented Whey at 121°C (250°F) under 15 psi weekly from 0 to 42 days. ASVs having less than 6
reads or present in only one
Blood samples were
for 15 minutes, then cooled
and stored at 5±1°C. A pure collected from wing veins on sample filtered out. Statistical
Protein in Broiler Chicken Diets culture of Limosilactobacillus day 42 (4 birds/replicate). analysis for hematological
and biochemical data
Hematological parameters
fermentum (M4, MF951096)
2
5
6
3
4
1
By Bhagyashree Das , Mansi Desai , Nikesh J. Bhagora , Prakash Koringa , Mohsin Pathan , J. C. Thakor , was inoculated into the (RBC, WBC, Hb, HCT, MCV, involved one-way ANOVA
followed by Duncan's test,
7
8
Fulabhai P. Savaliya , Shaikh Adil , and Subrota Hati 9 sterilized whey at 2.5% and MCH, MCHC) were analyzed
fermented at 37°C for 48 using a Mindray BC 2800 Vet with results expressed as
¹ & ⁸: Parul University, Vadodara
²: CHARUSAT Campus hours. FWP was extracted via hematology analyzer. Serum mean ± SEM at p<0.05.
³, ⁴, ⁵, ⁶, ⁷ & ⁹: Kamdhenu University centrifugation at 10,000 rpm biochemical parameters Results
Introduction by-product generated in immense for 30 minutes at 4°C. (glucose, cholesterol, LDL, Effect of fermented cheese
The poultry industry, a significant quantities (180–200 million metric Supernatants were HDL, VLDL, TG, TC) were whey fractions
global producer of chicken and eggs tons globally in 2023). ultrafiltered using Amicon determined using supplementation on growth
®
(with production and consumption This research introduces Fermented Milipore membranes with 10 commercial kits and an auto performance of broilers
elevating significantly over the past Whey Peptides (FWP), derived from kDa and 3 kDa cut-offs at analyzer. Histological analysis No significant (p < 0.05)
two decades), faces mounting lactic fermented cheddar cheese whey 5000 rpm for 30 minutes at involved preparing 5 mm x effect was observed on body
concerns from researchers and using Limosilactobacillus fermentum 4°C, yielding >10 kDa, <10 10 mm x 20 mm sections of weight gain at the 6th week
consumers alike regarding antibiotic (M4, GenBank Accession Number: kDa, and <3 kDa peptide intestine, liver, and heart, across all groups (Control T1,
resistance. The widespread historical MF951096). FWP leverages the fractions. Samples were fixed in 10% neutral buffered FWP-fed T2, T3, T4).
use of antibiotics as growth promoters nutritional richness of whey while stored at −20°C. formalin (NBF), processed, Numerically, the control
in food animals since the 1940s and offering a pre-digested, lactose-free, The in vivo study, adhering embedded in paraffin, group (T1: 2730.13±28.84 g)
1950s has unfortunately fostered the and bioactive-peptide-rich feed to CPCSEA and ARRIVE sectioned (5–6 μm) using a exhibited a higher average
emergence of antibiotic-resistant additive, readily available year-round. guidelines (Approval no. Microtome, and stained with body weight than FWP-fed
pathogens, posing risks to both Fermentation, conducted at 37°C for IAEC385/PRS/2022), involved Hematoxylin and Eosin (H & groups (T3: 2651.63±32.98 g;
animal and human health, including 48 hours, hydrolyzes whey proteins 96 one-day-old mixed-sex E). T4: 2707.03±51.34 g; T2:
gut microbiota disruption, into peptide fragments (3 kDa to 10 commercial broiler chicks For metagenomic analysis, 2626.38±40.74 g) as shown
hypersensitivity reactions, and kDa), enhancing bioavailability and (initial BW 56.07±3.21 g) cecal contents were collected in Fig. 1. However, FWP
potential carcinogenicity. This critical releasing bioactive compounds with from Venky's India. A post-euthanasia via cervical fractions did show a
challenge necessitates the demonstrated antioxidative, Completely Randomized dislocation, mixed 1:1 with significant (p < 0.05) impact
development of safe and effective antidiabetic, and anti-inflammatory Design (CRD) was employed, RNA protect Bacterial on body weight gain during
alternatives to conventional antibiotic properties. This study, involving 96 with four treatments, each reagent (Qiagen), and stored the 2nd, 3rd, and 5th weeks
growth promoters (AGPs). one-day-old mixed-sex commercial having four replicates of six at −20°C then −80°C. of the study. Specifically, at
One promising avenue lies in the broiler chicks in a Completely birds (6×4×4). Treatments Metagenomic DNA was the 4th week, the group
valorization of cheese whey, a dairy Randomized Design, investigated FWP were: T1 (Control: basal diet extracted using QIAamp Fast supplemented with <10 kDa

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